CSM Single Drop out formulation-csm-single:CSM, Single Drop-Out -Iso: 740 mg/l Synthetic Defined Minimal Media

Synthetic Defined Minimal Media

The hydroxyl group present at the 3 position of G-418 is phosphorylated by NPTII

Soluble in water (20°C

Friehs and Flaschel (2004)

30 H3BO3 1

CSM Single Drop out formulation-csm-single:CSM, Single Drop-Out -Iso: 740 mg/l Synthetic Defined Minimal MediaCSM is also a basis for many Drop Out mixtures to select for auxotrophic requirements and transformants. Each CSM Single Drop Out mixture contain all components except for one essential nutrient, i. e. the dropped out supplement. Some CSM Single Drop Out mixtures are also available with additions of extra Amino Acids and other components. Store all Drop out mixtures dry at room temperature